Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9748741 | Journal of Chromatography A | 2005 | 7 Pages |
Abstract
A size-exclusion chromatographic method using an aqueous solvent as eluent was developed for the determination of the molecular mass of procyanidins has been developed. Size-exclusion chromatography is a chromatographic technique used to determine molecular weight distribution of polymers. During the development work, PL⢠aquagel-OH columns and several different eluent compositions were investigated. The best separation of procyanidins was obtained with an eluent consisting of water-methanol (50:50, v/v), NaNO3: 0.18 M, pH < pKa + 2. The results from this aqueous SEC method were compared with results obtained using SEC with organic solvents.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
M.F. Nonier, N. Vivas, N. Vivas de Gaulejac, E. Fouquet,