Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9748804 | Journal of Chromatography A | 2005 | 8 Pages |
Abstract
A sensitive high-performance capillary zone electrophoresis (CZE) with head-column field-amplified sample stacking (FASS) in binary system has been developed for the simultaneous determination of zotepine and its active metabolite, norzotepine, in human plasma. The separation of zotepine and norzotepine was performed using a background electrolyte consisting of 50% ethylene glycol-borate buffer (20Â mM, pH 8.0) solution with 20% methanol as the running buffer and on-column detection at 200Â nm. Under the optimal FASS-CZE condition, good separation with high efficiency and short analysis time is achieved. Several parameters affecting the separation and sensitivity of the drug were studied, including sample matrix, pH and concentrations of the borate buffer, ethylene glycol and methanol. Using clozapine as an internal standard, the linear ranges of the method for the determination of zotepine and norzotepine in human plasma were over 3-100Â ng/mL; the detection limits of zotepine and norzotepine in plasma were 2 and 1Â ng/mL, respectively. A sample pretreatment by means of solid-phase extraction (SPE) with subsequent quantitation by FASS-CZE was used. The application of the proposed method for determination of zotepine and norzotepine in plasma collected after oral administration of 125Â mg zotepine in one schizophrenic patient was demonstrated.
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Authors
Yu-Wei Chou, Wei-Shan Huang, Cheng-Chung Chen, Shun-Jin Lin, Hsin-Lung Wu, Su-Hwei Chen,