Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9748890 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
A fast and highly sensitive ion chromatographic method using monolithic ODS columns was developed for the determination of nitrite (NO2â) and nitrate (NO3â) in seawater. Two monolithic ODS columns (50 mm Ã 4.6 mm i.d. + 100 mm Ã 4.6 mm i.d.) connected in series were coated and equilibrated with 5 mM cetyltrimethylammonium chloride (CTAC) aqueous solution. The column efficiency with 0.5 M NaCl as the mobile phase did not decrease in spite of the increase in flow rate of the mobile phase. Thus, good chromatograms were obtained within 3 minutes for NO2â and NO3â in artificial seawater without interferences by coexisting ions. The detection limit (S/N = 3) with UV detection at 225 nm was 0.8 and 1.6 μg/L for NO2â and NO3â, respectively. The characteristics of the monolithic CTA+-coated ODS columns were discussed. The present method was successfully applied to the fast and sensitive determination of NO2â and NO3â in real seawater samples.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kazuaki Ito, Yohichi Takayama, Nobuyuki Makabe, Ryo Mitsui, Takeshi Hirokawa,