Article ID Journal Published Year Pages File Type
9748893 Journal of Chromatography A 2005 7 Pages PDF
Abstract
A chiral stationary phase (CSP) based on (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid has been successfully employed in the liquid chromatographic resolution of eleven β-blockers containing a secondary amino functional group. As the result of an effort to find out the optimal mobile phase condition, the mixture of trifluoroacetic acid-triethylamine-ethanol-acetonitrile with the ratio of 0.1/0.5/20/80 (v/v/v/v) was concluded to be the best mobile phase condition, the separation (α) and the resolution factors (RS) for the resolution of 11 β-blockers being in the range of 1.13-1.85 and 1.36-5.79, respectively. Surprisingly, in contrast to the resolution of other racemic compounds containing a primary amino functional group, the separation factors (α) for the resolution of β-blockers were observed to improve as the column temperature increased and these unusual chromatographic behaviors were rationalized as the entropically controlled enantioselectivity.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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