| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9748956 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
NÉ-Monomethyllysine was identified in the serum, urine, brain, and liver samples of rats treated per os with l-deprenyl. The identification procedure included reaction with Fmoc chloride, clean-up, and analysis using HPLC-UV-MS. Oral administration of (â)-N-14C-methyl-N-propynyl(2-phenyl-1-methyl)ethylammonium hydrochloride l-deprenyl) to rats resulted in transfer of the radiolabelled methyl group to the NÉ-amino group of the endogenous lysine. The radiolabelled NÉ-monomethyllysine was urinary eliminated together with the other radiolabelled deprenyl metabolites, such as deprenyl-N-oxide and methamphetamine. The presence of NÉ-monomethyllysine has also been traced, and its concentrations were compared in the serum, liver and brain of rats subjected to l-deprenyl treatment. Methyl group transfer from the l-deprenyl to endogenous compounds; and the urinary elimination of their products may offer a vital way to eliminate or to decrease the degree of drug transmethylation to the lysine constituents of blood vessels' proteins.
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Authors
H. Kalász, Z. Szücs, M. Tihanyi, Á. Szilágyi, J. Lengyel,