Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749000 | Journal of Chromatography A | 2005 | 8 Pages |
Abstract
A simple and sensitive method for determining anatoxin-a in aqueous samples was developed using solid-phase microextraction (SPME) and gas chromatography with mass spectrometry (GC-MS) detection. Three forms of polyaniline (PANI) films and a single form of polypyrrole (PPY) film were prepared and applied for SPME. The extraction properties of these films to anatoxin-a were examined and it was shown that leucoemeraldine form of PANI displayed a better selectivity to this compound. SPME conditions were optimized by selecting the appropriate extraction parameters, including type of coating (leucoemeraldine form of PANI at 32 μm thicknesses), salt concentration (10%, w/v), time of extraction (30 min) and stirring rate (1000 rpm). The calibration curve was linear in the range from 50 to 10 000 ng/ml, with the detection limit (S/N = 3) of 11.2 ng/ml. This method was successfully applied for the analysis of anatoxin-a in the cultured media of two species of cyanobacteria.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Alireza Ghassempour, Nahid Mashkouri Najafi, Ali Mehdinia, Saied Saeed Hosseiny Davarani, Maryam Fallahi, Maryam Nakhshab,