Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749027 | Journal of Chromatography A | 2005 | 6 Pages |
Abstract
We present a liquid chromatographic-mass spectrometric assay for the simultaneous determination of sulfadoxine and pyrimethamine in human plasma samples. Sample clean-up was achieved by adding acetonitrile for protein precipitation. Gradient elution in only 10Â min resulted in high throughput capability. Tandem mass spectrometric detection in multiple reaction monitoring was used for quantification. The developed analytical approach was successfully validated and was applied in the pharmacokinetic evaluation of the bioavailability between two sulfadoxine/pyrimethamine formulations available on the Eastern African market, using a cross-over design.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bart A. Sinnaeve, Tineke N. Decaestecker, Peter G. Risha, Jean-Paul Remon, Chris Vervaet, Jan F. Van Bocxlaer,