Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749029 | Journal of Chromatography A | 2005 | 8 Pages |
Abstract
An automated method using solid-phase extraction (SPE) for the concentration and clean-up of soy isoflavone extracts is proposed in this work. Using a standardized sample (0.1Â g of a freeze dried soybean extract/25Â mL of water); eight SPE cartridges with a wide range of sorbents (C18, divinylbenzene and modified divinylbenzene) from different suppliers were evaluated and compared. A large variation on SPE cartridges performance was observed, especially regarding retention and breakthrough volume of isoflavones during sample load and washing steps. The most effective cartridges were the divinylbenzene based cartridges, especially Strata X (from Phenomenex) and HLB oasis (from Waters). Using Strata X cartridges, several extraction parameters, such as sample loading flow (5-15Â mLÂ minâ1), extracting solvent volume (2-6Â mL of methanol), pH of the extracting solvent and the necessity of drying the sorbent before elution, were evaluated to provide a fast, specific, quantitative and reproducible SPE method. The optimized method consists of conditioning the cartridge with 10Â mL of methanol and 10Â mL of water (10Â mLÂ minâ1), loading 25Â mL of the standardized extract onto the cartridges (5Â mLÂ minâ1), washing the cartridge with 10Â mL of water (10Â mLÂ minâ1) and finally eluting with 4Â mL of methanol (10Â mLÂ minâ1). Mean isoflavones recovery was 99.37% and mean intra- and inter-day reproducibility was higher than 98%. The developed sample clean-up/concentration (6.25:1) method takes less than 10Â min and can be used in the analysis of isoflavones from soy extracts.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mauricio A. Rostagno, Miguel Palma, Carmelo G. Barroso,