| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9749040 | Journal of Chromatography A | 2005 | 4 Pages |
Abstract
A simple and rapid preparative high-performance liquid chromatography (HPLC) method has been developed to isolate and characterize some minor impurities of astaxanthin using a normal-phase Lichrosorb silica column with n-hexane-acetone-tetrahydrofuran (90:2:8, v/v/v) as mobile-phase and detection at 475Â nm. The isolated impurities were characterized as astacene, dehydro astacene and apoastaxanthinal by UV-vis, ESI-MS, 1H and 13C NMR spectroscopy and the molecular structures were assigned. The impurities collected using the developed conditions were over 98% pure.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
R. Nageswara Rao, S. Naseeruddin Alvi, B. Nageswara Rao, HPLC Group HPLC Group,