Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749067 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
Four flavonoids, apigenin-7-O-neohesperidoside, luteolin-7-O-glucoside, apigenin-7-O-glucoside and kaempferol-7-O-glucoside have been isolated and purified for the first time from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography with a two-phase solvent system composed of ethyl acetate-ethanol-acetic acid-water (4:1:0.25:5, v/v). Then, 5Â mg apigenin-7-O-neohesperidoside, 4Â mg luteolin-7-O-glucoside, 9Â mg apigenin-7-O-glucoside and 2.5Â mg kaempferol-7-O-glucoside could be obtained after injecting 40Â mg sample and their purities were 94, 97, 97 and 96%, respectively. All these constituents were identified by mass spectrometry and nuclear magnetic resonance.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiao Wang, Chuange Cheng, Qinglei Sun, Fuwei Li, Jianhua Liu, Chengchao Zheng,