Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749089 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
We have previously established a rapid catechol-O-methyltransferase (COMT) assay using norepinephrine (NE) as a natural substrate and flow-injection analysis. In this study, the method is improved for screening of COMT inhibitors or activators using reversed-phase high-performance liquid chromatographic separation with fluorescence detection. The excess substrate, NE, was removed by the addition of borate in the eluent for HPLC to make an ionic complex with NE, which was eluted faster than the enzymatic product, normetanephrine. The method had good precision and accuracy, and was able to assay one sample in 5Â min, showing the usability for screening of COMT inhibitors or activators.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nozomi Aoyama, Makoto Tsunoda, Kazuhiro Imai,