Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749097 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
High-speed counter-current chromatography (HSCCC) with a solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v) was used to isolate and separate two C-glycosylflavones from Patrinia villosa Juss, a traditional Chinese medicine. The separation produced 42.9Â mg isovitexin and 20.1Â mg isoorientin with purities of 99.3% and 98.5%, respectively as determined by high-performance liquid chromatography (HPLC) in one step elution from 250Â mg crude extract, and identification was performed by MS, 1H NMR and 13C NMR. It is the first report of discovering isovitexin and isoorientin from the plant of Patrinia genus.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jinyong Peng, Guorong Fan, Zhanying Hong, Yifeng Chai, Yutian Wu,