Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749100 | Journal of Chromatography A | 2005 | 6 Pages |
Abstract
High-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water system (5:5:7:5, v/v) was applied to the isolation and purification of alkaloids from the Chinese medicinal plant Evodia rutaecarpa (Juss.) Benth. Five kinds of alkaloids were obtained and yielded 28Â mg of evodiamine (I), 19Â mg of rutaecarpine (II), 21Â mg of evocarpine (III), 16Â mg of 1-methy-2-[(6Z,9Z)]-6,9-pentadecadienyl-4-(1H)-quinolone (IV), 12Â mg of 1-methyl-2-dodecyl-4-(1H)-quinolone (V) from 180Â mg of crude extract in a one-step separation, with the purity of 98.7%, 98.4%, 96.9%, 98.0%, 97.2%, respectively, as determined by high performance liquid chromatography (HPLC). The structures of these compounds were identified by 1H NMR and 13C NMR.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Renmin Liu, Xin Chu, Ailing Sun, Lingyi Kong,