Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749119 | Journal of Chromatography A | 2005 | 6 Pages |
Abstract
We describe a novel two-dimensional liquid chromatography (2D-LC) method for fast and robust isolation and concentration of low abundant carbohydrates (sorbitol, glycerol) from biological matrices (plasma and urine). Off-line pre-purified fractions, enriched by analyte of interest, were analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS-MS). Initial 2D-LC automated sample pre-purification improved MS detection, eliminated matrix effects, and achieved high sensitivity (picogram detection limit) with a 6Â min runtime and increased column lifetime. Using this method we have analyzed more than 1300 samples from biological matrices without column replacement.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Eduard Rogatsky, Daniel Stein,