Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749263 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
Cholesterol oxidation products (COPs) can be formed in the body or in animal foods from cholesterol during food processing. A new method for the extraction and quantification of cholesterol, 7-ketocholesterol, cholestane-3β-5α-6β-triol, 25-hydroxycholesterol, 5,6α-epoxycholesterol, and 7β-hydroxycholesterol by means of reversed-phase LC/atmospheric pressure chemical ionization mass spectrometry is presented. A baseline separation of all COPs was achieved, allowing a separate quantification also for isobaric compounds. The limits of detection were 15-30 ng/mL, quantification was performed from 100 ng/mL to 10 μg/mL with RSD < 2%. The method was applied successfully to the determination of cholesterol and COPs in processed foods such as pork, beef, chicken, and egg.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Klaus Raith, Christian Brenner, Hany Farwanah, Gerit Müller, Klaus Eder, Reinhard H.H. Neubert,