| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9749337 | Journal of Chromatography A | 2005 | 9 Pages |
Abstract
Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1:1, v:v). By injecting 200Â mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165Â mg of acteoside and 17.5Â mg of isoacteoside from 978Â mg extract. The recovery rates for acteoside and isoacteoside were 90 and 84%, respectively, and the purities were 98 and 94%, respectively. The HSCCC fractions were analyzed by HPLC and the structures were identified by UV, LC-APCI-MS in negative ion mode, and confirmed by NMR experiments.
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Authors
Li Li, Rong Tsao, Zhiqiang Liu, Shuying Liu, Raymond Yang, J. Christopher Young, Honghui Zhu, Zeyuan Deng, Mingyong Xie, Zhihong Fu,