Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749346 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
Analytical Milli high-speed counter-current chromatography (HSCCC) was used for the selection and optimization of the two-phase solvent system to separate flavonoids from the extracts of the seeds of Oroxylum indicum. The optimum solvent system obtained from Milli-CCC was also the best solvent system for preparative HSCCC and led to the successful separation of two crude flavonoids from the seeds of O. indicum by Lab/Prep (laboratory preparative) HSCCC using different sized coils. Four flavonoids were isolated by preparative HSCCC: baicalein-7-O-diglucoside (25.0Â mg, 92% purity), baicalein-7-o-glucoside (50.4Â mg; 95% purity), baicalein (75Â mg; purity 98%) and chrysin (100Â mg; purity 98%).
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
L.J. Chen, H. Song, X.Q. Lan, D.E. Games, I.A. Sutherland,