Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749347 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
Following an initial clean-up step on the AB-8 resin (polystyrene resin, 0.3-1.25Â mm: NanKai Chemical Factory, Tianjin, China), high-speed counter-current chromatography (HSCCC) was used to purify an arctiin from an extract of the fruits of the Arctium lappa L. Arctiin is a major lignan compound in the traditional Chinese medicinal herb A. lappa L. The two-phase solvent system used was composed of ethyl acetate-n-butanol-ethanol-water at an optimized volume ratio of 5:0.5:1:5 (v/v/v/v). The upper phase was used as the mobile phase in the head to tail elution mode. A total amount of 159Â mg of arctiin at 98% purity was obtained from 350Â mg of the crude extract (containing 49% arctiin) with 91% recovery. The preparative isolation and purification of arctiin by HSCCC was completed in 5Â h in a separation. Identification of the target compound was performed by LC-electrospray ionization MS and 13C-NMR. The structure of the product was further confirmed by comparison with authentic sample (National Institute of the Control of Pharmaceutical and Biological Products, Beijing, China).
Related Topics
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Authors
Xiao Wang, Fuwei Li, Qinglei Sun, Jingpeng Yuan, Ting Jiang, Chengchao Zheng,