Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9749348 | Journal of Chromatography A | 2005 | 4 Pages |
Abstract
We have developed a new microfluidic chip capable of accurate metering, pneumatic sample injection, and subsequent electrophoretic separation. The pneumatic injection scheme, enabling us to introduce a solution without sampling bias unlike electrokinetic injection, is based upon the hydrophobicity and wettability of channel surfaces. An accurately metered solution of 10Â nL could be injected by pneumatic pressure into a hydrophilic separation channel through Y-shaped hydrophobic valves, which consist of polydimethylsiloxane (PDMS) and fluorocarbon (FC) film layers. We demonstrated the successful pneumatic injection of a red ink solution into the separation channel as a proof of the concept. A mixture of fluorescein and dichlorofluorescein (DCF) could be baseline-separated using a single power source in microchip electrophoresis.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Seung Il Cho, Sang-Ho Lee, Doo Soo Chung, Yong-Kweon Kim,