Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9753166 | Journal of Chromatography B | 2005 | 8 Pages |
Abstract
A poly (methacrylic acid-ethylene glycol dimethacrylate, MAA-EGDMA) monolithic capillary was used for the in-tube solid-phase microextraction (in-tube SPME) of several angiotensin II receptor antagonists (ARA-IIs) from human plasma and urine. Under the optimized extraction condition, the protein component of the biological sample was flushed through the monolithic capillary, while the analytes were successfully trapped. Coupled to HPLC with fluorescence detection, this on-line in-tube SPME method was successfully applied for the determination of candesartan, losartan, irbesartan, valsartan, telmisartan, and their detection limits were found to be 0.1-15.3Â ng/mL and 0.1-15.2Â ng/mL in human plasma and urine, respectively. The method was linear over the range of 0.5-200Â ng/mL for telmisartan, 5-2000Â ng/mL for candesartan and irbesartan, 10-2000Â ng/mL for valsartan, and 50-5000Â ng/mL for losartan with correlation coefficients being above 0.9985 in plasma sample and above 0.9994 in urine sample. The method reproducibility was evaluated at three concentration levels, resulting in the R.S.D. <7%. The poly (MAA-EGDMA) monolithic capillary was demonstrated to be robust and biocompatible by using direct injections of biological samples.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jing Nie, Min Zhang, Yi Fan, Yi Wen, Bingren Xiang, Yu-Qi Feng,