Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9753167 | Journal of Chromatography B | 2005 | 5 Pages |
Abstract
Glabridin is a major flavonoid included specifically in licorice (Glycyrrhiza glabra L.), and has various physiological activities including antioxidant and anti-inflammatory effects. We have developed and validated an analytical method for determination of glabridin in human plasma by solid-phase extraction (SPE) and LC-MS/MS. Glabridin was extracted from plasma by SPE using a C8 cartridge and analyzed by LC-MS/MS using mefenamic acid as an internal standard (IS). The analyte were separated by a C18 column on LC, and monitored with a fragment ion of m/z 201 formed from a molecular ion of m/z 323 for glabridin and that of m/z 196 from m/z 240 for IS during negative ion mode with tandem MS detection. The lower limit of quantitation (LLOQ) of glabridin was 0.1 ng/mL in plasma, corresponding to 1.25 pg injected on-column. The calibration curves exhibited excellent linearity (r > 0.997) between 0.1 and 50 ng/mL. Precision and accuracy were <17 and <±7% at LLOQ, and <11 and <±5% at other concentrations. Glabridin was recovered >90%, and was stable when kept at 10 °C for 72 h, at â20 °C until 12 weeks, and after three freeze-thaw cycles. This is the first report on determination of glabridin in body fluids by the selective, sensitive, and reproducible method.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Fumiki Aoki, Kaku Nakagawa, Akiyoshi Tanaka, Kazue Matsuzaki, Naoki Arai, Tatsumasa Mae,