| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9753176 | Journal of Chromatography B | 2005 | 4 Pages |
Abstract
A modification of the Bio-Rad® total homocysteine HPLC-test is presented in order to enable not only plasma homocysteine measurements but also the quantification of homocysteine in urine samples using the same principle of measurement. Coelution of the internal standard provided in the test kit with an endogenous compound in urine demands for an alternative analytical procedure. Therefore, we introduced 3-mercaptopropionic acid as a substitute for the internal standard. The analytical method validation was performed for the matrix of urine specimens. The applicability of this method was demonstrated in a clinical study with volunteers after homocysteine thiolactone hydrochloride loading.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Barbara Proksch, Simone Jelesnianski, Walter Oberrauch, Richard Fux, Christoph H. Gleiter,