Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9753227 | Journal of Chromatography B | 2005 | 9 Pages |
Abstract
Evaluation of different extraction methods for quantification of endogenous sorbitol and fructose in human red blood cells (RBCs) and matrix effects in ESI and APCI showed that protein-precipitation followed by mixed-mode solid-phase extraction was more effective extraction method and APCI more effective ionization method. Then the LC/APCI-MS/MS method was fully validated and successfully applied to analysis of clinical RBC samples. The concentrations of endogenous sorbitol and fructose were determined using calibration curves employing sorbitiol-13C6 and fructose-13C6 as surrogate analytes. The method has provided excellent intra- and inter-assay precision and accuracy with a linear range of 50.0-10,000Â ng/mL (correlation coefficient >0.999) for sorbitol-13C6 and 250-50000Â ng/mL (correlation coefficient >0.999) for fructose-13C6 in human RBCs.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
H.R. Liang, T. Takagaki, R.L. Foltz, P. Bennett,