Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9753236 | Journal of Chromatography B | 2005 | 9 Pages |
Abstract
17α-Methyltestosterone (MT) is used to manipulate the gender of a variety of fish species. A high performance liquid chromatography (HPLC) internal standard method for the determination of 17α-methyltestosterone in fish feed using 3β-methoxy-17β-hydroxyandrost-5-en-7-one as internal standard (IS) has been developed. The method has been validated for the quantitation of MT in fish feed using 245 nm UV absorbance as the parent wavelength and 255 nm as a qualifier wavelength. The method was validated in the concentration range of 15.0-120 mg/kg of 17α-methyltestosterone in fish feed. Method was also found to be suitable for other feeds.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ashok Marwah, Padma Marwah, Henry Lardy,