Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9753237 | Journal of Chromatography B | 2005 | 7 Pages |
Abstract
Increase in methylglyoxal is thought to be involved in different pathological conditions. Deamination of aminoacetone by semicarbazide-sensitive amine oxidase (SSAO) leads to production of methylglyoxal. We have synthesized aminoacetone and developed a novel HPLC procedure for its quantitative determination. The urinary excretion of aminoacetone is approximately 20-30 μg/mouse/day, and the concentration is about 0.5 μg/g in mouse liver and small intestine. SSAO inhibitor increases aminoacetone levels in both tissues and urines. Results confirm that aminoacetone is an endogenous substrate for SSAO. However, data also indicate that deamination is not the only catabolic pathway for aminoacetone.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Michael Kazachkov, Peter H. Yu,