| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9753256 | Journal of Chromatography B | 2005 | 9 Pages |
Abstract
A quantitative method for measuring testosterone (T) concentrations in rat plasma was developed using ethyl oxime and acetyl ester derivatization and liquid chromatography-atmosphere pressure chemical ionization tandem mass spectrometry (LC-APCI-MS/MS). The method utilizes a solid phase extraction with Varian Bond Elut C18, a derivatization process to form testosterone ethoxime acetate and LC-APCI-MS/MS with a reversed phase LC and a C8 column. This method is capable of detecting testosterone concentrations as low as 0.2Â ng/ml in a 0.05Â ml sample of rat plasma. This method can be used as a sensitive chromatography-based assay for small sample volumes of rat blood.
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Authors
Makoto Niwa, Noriko Watanabe, Hisashi Ochiai, Kouwa Yamashita,