| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9753308 | Journal of Chromatography B | 2005 | 6 Pages |
Abstract
An accurate, sensitive, reproducible, and selective liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for determination of aripiprazole and its main metabolite, OPC-14857, in human plasma was developed and validated. Chromatographic separation was achieved isocratically on a C18 reversed-phase column within 7.5Â min. The calibration curve, ranging from 0.1 to 100Â ng/ml, was fitted to a 1/y2-weighted linear regression model. The assay showed no significant interference. Lower limit of quantitation (LLOQ) for both analytes was 0.1Â ng/ml using 0.4Â ml of plasma. Intra- and inter-assay precision and accuracy values for aripiprazole and OPC-14857 were within regulatory limits.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Masanori Kubo, Yasuo Mizooku, Yukihiro Hirao, Takahiko Osumi,