Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9753314 | Journal of Chromatography B | 2005 | 4 Pages |
Abstract
A sensitive and selective HPLC method with UV detection (290Â nm) was developed and validated for quantitation of pantoprazole, proton-pump inhibitor, in human plasma. Following a single-step liquid-liquid extraction with methyl tert-butyl ether/diethyl ether (70/30, v/v), the analyte and internal standard (zonisamide) were separated using an isocratic mobile phase of 10Â mM phosphate buffer (pH 6.0)/acetonitrile (61/39, v/v) on reverse phase Waters symmetry® C18 column. The lower limit of quantitation was 20Â ng/mL, with a relative standard deviation of less than 4%. A linear range of 20-5000Â ng/mL was established. This HPLC method was validated with between-batch and within-batch precision of 1.3-3.2% and 0.7-3.3%, respectively. The between-batch and within-batch bias was â0.5 to 8.2 % and â2.5 to 12.1%, respectively. This validated method is sensitive and repeatable enough to be used in pharmacokinetic studies.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
N.V.S. Ramakrishna, K.N. Vishwottam, S. Wishu, M. Koteshwara,