Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9753355 | Journal of Chromatography B | 2005 | 6 Pages |
Abstract
5-Hydroxytryptophol glucuronide (GTOL) is the major excretion form of 5-hydroxytryptophol (5-HTOL), a minor serotonin metabolite under normal conditions. Because the concentration of 5-HTOL is markedly increased following consumption of alcohol, measurement of 5-HTOL is used as a sensitive biomarker for detection of recent alcohol intake. This study describes the development and evaluation of a liquid chromatography-electrospray ionization mass spectrometry (LC-MS) procedure for direct quantification of GTOL in human urine. Deuterium labelled GTOL (GTOL-2H4) was used as internal standard. GTOL was isolated from urine by solid-phase extraction on a C18 cartridge prior to injection onto a gradient eluted Hypurity C18 reversed-phase HPLC column. The detection limit of the method was 2.0 nmol/L and the measuring range 6-8500 nmol/L. The intra- and inter-assay coefficients of variation were <3.5% (n = 10) and <6.0% (n = 9), respectively. The new LC-MS method was highly correlated with an established GC-MS method for urinary 5-HTOL (r2 = 0.99, n = 70; mean 5-HTOL/GTOL ratio = 1.10). This is the first direct assay for quantification of GTOL in urine. The method is suitable for routine application.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nikolai Stephanson, Helen Dahl, Anders Helander, Olof Beck,