Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9753360 | Journal of Chromatography B | 2005 | 7 Pages |
Abstract
A highly sensitive high-performance liquid chromatographic-tandem mass spectrometric method (HPLC-MS-MS) has been developed to quantitate clemastine in human plasma for the purpose of pharmacokinetic studies. Sample preparation was carried out by liquid-liquid extraction using deuterated clemastine as an internal standard. Chromatographic separation used a C18 reversed phase polymer column giving an extremely fast total run time of 2 min. The method was validated and used for the bioequivalence study of clemastine tablets in healthy male volunteers (n = 28). The lower limit of detection proved to be 0.01 ng/ml for clemastine.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Viola Horváth, Antal Tolokán, Andrea Egresi, TÃmea Pap, George Horvai, Katalin Balogh-Nemes, Imre Klebovich,