Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9753383 | Journal of Chromatography B | 2005 | 5 Pages |
Abstract
An HPLC-UV analytical method for estimation of iohexol in human plasma was developed and validated. Protein precipitation and iohexol extraction from plasma (100 μl) was carried out by adding 800 μl perchloric acid (5%, v/v in water) containing iohexol related compound B as the internal standard followed by vortex mixing and centrifugation. The supernatant (90 μl) was then injected onto a μBondapak C18 column (150 mm Ã 3.9 mm, 10 μm) maintained at 30 °C. The mobile phase comprised of various proportions of acetonitrile and water with a total run time of 12 min and the wavelength of the UV detector was set at 254 nm. The extraction recovery of iohexol from plasma was >95% and the calibration curve was linear (r2 = 0.99) over iohexol concentrations ranging from 10 to 750 μg/ml (n = 8). The method had an accuracy of >92% and intra- and inter-day CV of <3.7% and <3.6%, respectively. The method reported is simple, reliable, precise, accurate and has the capability of being used for determination of iohexol in clinical settings.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Rohit S. Soman, Hamim Zahir, Fatemeh Akhlaghi,