Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9754493 | Journal of Pharmaceutical and Biomedical Analysis | 2005 | 6 Pages |
Abstract
A major impurity associated with primaquine drug samples obtained from European Pharmacopoeia (EP) and other commercial sources was detected and identified using HPLC, photo diode array (PDA), LC-MS/MS and gas chromatograph-electron impact-mass spectrometer (GC-EI-MS). PDA and LC-ESI-MS/MS data provided an evidence for it being isomeric in nature. However, spectral data obtained from the newly developed GC-EI-MS method has been utilised for structural elucidation and found to be conclusive to characterize this impurity as positional isomer, i.e. 8-(4-amino-4-methylbutyl amino)-6 methoxyquinoline. The structure of this impurity has been confirmed by its synthesis. Precursor of primaquine was also investigated using GC-EI-MS. The data obtained confirmed the origin of isomeric impurity in precursor.
Keywords
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Vaijanath G. Dongre, Pravin P. Karmuse, M.M. Nimbalkar, Dharmendra Singh, Ashok Kumar,