| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9754986 | Microchemical Journal | 2005 | 5 Pages |
Abstract
Hydrophilic polymers, hydroxypropylmethyl cellulose (HPMC) and polyethylene oxide (PEO), were employed both for the dynamic coating of the capillary surface and for the sieving matrix. Although PEO showed somewhat better coating efficiency, it was found that both HPMC and PEO as the dynamic coating polymers were not able to maintain the capillary surface coating during the separation of deoxyribonucleic acid (DNA) fragments. However, the continuous supply of PEO chain from the bulk made it possible to maintain the surface coating. An interesting result was obtained with DNA intercalating dye, ethidium bromide (EB). Better resolution was obtained with the use of EB probably due to the change of radius of gyration (Rg) of DNA.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Daechun Kang, Doo Soo Chung, Seong Ho Kang, Yongseong Kim,