Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9755000 | Microchemical Journal | 2005 | 8 Pages |
Abstract
An integrated gold-disk microelectrode (IGME) was fabricated and modified with Fe(II)-phthalocyanine (Fe(II)-PC) for NO detection in biological media. Microanalysis of NO using square wave anodic stripping voltammetry (SWASV) in 0.01 M HClO4 was optimal at the initial potential of 0.1 V, frequency of 100 Hz, pulse amplitude of 25 mV, and a scan rate of 200 mV/s. When the electrode was modified with Fe(II)-phthalocyanines, the anodic peak current and sensitivity of NO were remarkably increased due to the catalytic oxidation of NO. The calibration curve had good linearity in the range from 3.6Ã10â5 to 7.2Ã10â7 M, and the detection limit was (5.7±1.2)Ã10â7 M. Fe(II)-phthalocyanine modified gold-disk microelectrode coated with Nafion was applied to determination of NO released from macrophage cell.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Il Kwang Kim, Hun Taeg Chung, Gi Soo Oh, Hyun Ok Bae, Sung Hyun Kim, Hyun Ja Chun,