Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9755023 | Microchemical Journal | 2005 | 5 Pages |
Abstract
Determination of ochratoxin A (OA) in musts and wines is described using high performance liquid chromatography with fluorescence detection (HPLC-FL). The applicability of common solid phase extraction cartridges with octadecyl-silica stationary phase (C18-SPE) instead of immunoaffinity columns (IAC) for sample clean-up and preconcentration of OA is discussed. Utilizing 100-fold concentration, the detection limit and quantification limit of OA in wine were 0.024 and 0.125 ng/ml, respectively. Mean recovery amounted to 83.5%. Confirmation of the presence of OA was performed by quantitative conversion of OA into its methyl ester with BF3/methanol. The method was successfully applied to routine analysis of 87 Hungarian must and wine samples. The method proved to be robust during the 2 weeks of routine measurements resulting in a mean recovery of 99.4% (std. dev. 2.4%, n=11) related to the first measurements on the control sample. OA was not detectable in the Hungarian wine and must samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bálint Berente, Ágnes Móricz, Klára H.-Otta, Gyula Záray, László Lékó, László Rácz,