Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9757012 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2005 | 8 Pages |
Abstract
The usefulness of derivative spectrophotometry for simultaneous assay of l-dopa, benserazide and ascorbic acid in pharmaceuticals was studied. The parameters of derivatisation depends on composition of solution in which particular compound was determined. For quantification of l-dopa in mixtures with benserazide or ascorbic acid the first derivative was used. Its determination in ternary mixture (l-dopa + benserazide + ascorbic acid) is possible by third derivative spectra. Benserazide was assayed in presence of l-dopa using first derivative while in ternary mixture by third derivative. Direct determination of ascorbic acid is possible applying first derivative only in presence of l-dopa. The elaborated derivative spectrophotometric methods were used for assaying of l-dopa and benserazide in their commercial form “Madopar”. The proposed spectrophotometric derivative method of simultaneous determination of l-dopa and benserazide was combined with FIA technique.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Joanna KarpiÅska, Jerzy Smyk, Elżbieta WoÅyniec,