Article ID Journal Published Year Pages File Type
9757012 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2005 8 Pages PDF
Abstract
The usefulness of derivative spectrophotometry for simultaneous assay of l-dopa, benserazide and ascorbic acid in pharmaceuticals was studied. The parameters of derivatisation depends on composition of solution in which particular compound was determined. For quantification of l-dopa in mixtures with benserazide or ascorbic acid the first derivative was used. Its determination in ternary mixture (l-dopa + benserazide + ascorbic acid) is possible by third derivative spectra. Benserazide was assayed in presence of l-dopa using first derivative while in ternary mixture by third derivative. Direct determination of ascorbic acid is possible applying first derivative only in presence of l-dopa. The elaborated derivative spectrophotometric methods were used for assaying of l-dopa and benserazide in their commercial form “Madopar”. The proposed spectrophotometric derivative method of simultaneous determination of l-dopa and benserazide was combined with FIA technique.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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