Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9757084 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2005 | 9 Pages |
Abstract
We report here the use of near-infrared (NIR) Fourier transform (FT) Raman spectroscopy to analyze normal human epidermal keratinocytes prior to and following malignant transformation. Our analysis indicates specific Raman spectral differences between immortalized (HPK1A) and malignant ras transformed (HPK1A-ras) cells. In addition, striking spectral differences are seen in the DNA isolated from these cells and particularly in the 843/810 cmâ1 ratio with values of 1.6 ± 0.13 in HPK1A cells and 0.68 ± 0.09 in HPK1A-ras cells (mean ± S.D., n = 12, P < 0.001) indicating specific alterations in the backbone conformation markers following malignant transformation. Subsequently, we analysed the effect of a strong inhibitor of keratinocyte growth, the Vitamin D analog EB1089, on the Raman spectra of intact cells and on the 843/810 cmâ1 ratio in the DNA isolated from both cell lines. Specific changes were observed in intact cells in the 1300-750 cmâ1 region. Furthermore, the 843/810cmâ1 ratio of isolated DNA from HPK1A cells was not affected by EB1089 but significantly increased in DNA isolated from HPK1A-ras cells so much that it became closer to the value observed for HPK1A cells (1.07 ± 0.10). Our data suggest that Raman analysis of DNA and in particular the 843/810cmâ1 ratio can provide useful indices of malignant transformation and efficacy of anticancer agents.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiaoling Gao, Ian S. Butler, Richard Kremer,