Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9757095 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2005 | 8 Pages |
Abstract
New terbium complexes of derivatives of 2-oxo-4-hydroxy-quinoline-3-carboxylic acid are reported, which are highly luminescent, water soluble and do not require luminescence enhancers. The triplet-state energy levels of the ligands, the relative quantum yields (QYs) and the excitation maxima of the respective terbium chelates were determined. The large luminescence enhancement of one of these complexes by nucleic acids was investigated and a mechanism of its interaction with DNA is proposed. The optimal conditions for determination of DNA are equal concentrations of Tb3+ and ligand R1 (C=1Ã10â6 M), pH 9.0. Under optimal conditions the luminescence intensity (RI) is proportional to the concentration of fish sperm DNA (fsDNA) or calf thymus DNA (ctDNA), respectively, within the range of 0.05-1.5 μg mlâ1. The detection limits were 10 ng mlâ1 for fsDNA and 12 ng mlâ1 for ctDNA.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Alla Yegorova, Alexander Karasyov, Axel Duerkop, Igor Ukrainets, Valery Antonovich,