Article ID Journal Published Year Pages File Type
9757471 Vibrational Spectroscopy 2005 7 Pages PDF
Abstract
The objective of the present study was to establish the conditions for infrared spectroscopy of single living cells in cell culture under aqueous media, using a high brilliance synchrotron light source to achieve acceptable S/N at the necessary high spatial resolution. This is experimentally challenging, but brings a number of significant advantages. The data presented show that the infrared spectra of single living cells can be monitored over several hours with high reproducibility, as well as confirming that acquisition of spectral data from single cells can contribute new insights that are not available from measurements of cell populations.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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