Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9774582 | Journal of Controlled Release | 2005 | 13 Pages |
Abstract
We here compare the efficacy of three different delivery strategies for antisense PNA: 1) conjugation to hydrophobic peptides, 2) adsorption onto polymeric microspheres and 3) encapsulation in autologous erythrocytes. To this purpose, we designed and prepared PNA sequences able to inhibit the expression of macrophage enzymes involved in inflammatory process, i.e. nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) and tested their antisense activity in a murine macrophage cellular model. Both delivery through polymeric microspheres and encapsulation into erythrocytes allowed the antisense activity of unmodified PNAs at nanomolar concentration.
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Physical Sciences and Engineering
Materials Science
Biomaterials
Authors
Laura Chiarantini, Aurora Cerasi, Alessandra Fraternale, Enrico Millo, Umberto Benatti, Katia Sparnacci, Michele Laus, Marco Ballestri, Luisa Tondelli,