Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9774633 | Journal of Controlled Release | 2005 | 12 Pages |
Abstract
ATP-loaded liposomes (ATP-L) infused into Langendorff-instrumented isolated rat hearts protect the mechanical functions of the myocardium during ischemia/reperfusion. The left ventricular developed pressure (LVDP) at the end of the reperfusion in the ATP-L group recovered to 72% of the baseline (preservation of the systolic function) compared to 26%, 40%, and 51% in the groups treated with Krebs-Henseleit (KH) buffer, empty liposomes (EL), and free ATP (F-ATP), respectively. The ATP-L-treated group also showed a significantly lower left ventricular end diastolic pressure (LVEDP; better preservation of the diastolic function) after ischemia/reperfusion than controls. After incubating the F-ATP and ATP-L with ATPase, the protective effect of the F-ATP was completely eliminated because of ATP degradation, while the protective effect of the ATP-L remained unchanged. Fluorescence microscopy confirmed the accumulation of liposomes in ischemic areas, and the net ATP in the ischemic heart increased with ATP-L. Our results suggest that ATP-L can effectively protect myocardium from ischemic/reperfusion damage.
Keywords
Krebs–HenseleitCPPFITC-dextranLVSPLVEDPLVDPadenosine-5′-triphosphateATPIschemiaATPaseleft ventricleLeft ventricular developed pressureEPRReperfusioncoronary perfusion pressureleft ventricular systolic pressureleft ventricular end diastolic pressureIsolated rat heartLiposomesEnhanced permeability and retentionPoly(ethylene glycol)PEG
Related Topics
Physical Sciences and Engineering
Materials Science
Biomaterials
Authors
D.D. Verma, T.S. Levchenko, E.A. Bernstein, V.P. Torchilin,