Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9776395 | Synthetic Metals | 2005 | 4 Pages |
Abstract
Alcohol dehydrogenase (ADH) was immobilized on the film of poly[1-(2-carboxyethy] pyrrole], which was prepared by electrochemical polymerization of 1-(2-carboxyethyl)pyrrole. The film exhibited a stable conductivity of 10â4-10â3 S/cm over the temperature range 0-200 °C. A significant amount of ADH (50-100 g/cm2) was immobilized on the polymer film by use of a condensing reagent. Although activity of ADH was decreased by the immobilization, the ADH-immobilized polymer film showed amperometric response to ethanol at +0.85 V vs. SCE in the presence of nicotinamide adenine dinucleotide (a redox mediator). These results demonstrate that the polymer film acts as both an ADH-supporting layer and a conductive medium in the alcohol-driven amperometric device.
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Authors
Kenji. Oshima, Taichi. Nakamura, Ryoko. Matsuoka, Takashi. Kuwahara, Masato. Shimomura, Sinnosuke. Miyauchi,