Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9786199 | Optics Communications | 2005 | 13 Pages |
Abstract
We show that automatic determination of regularization threshold and pre-filtering of 3-D fluorescence microscopic images improves the stability of deconvolution results when using the Linear Least squares Solution or the Maximum a Posteriori method. Doing so, the choice of the regularization parameter much less depends on a priori knowledge of the specimen or skills of the operator. This increases the reliability and repeatability of quantitative measurements on deconvolved images.
Related Topics
Physical Sciences and Engineering
Materials Science
Electronic, Optical and Magnetic Materials
Authors
B. Colicchio, O. Haeberlé, C. Xu, A. Dieterlen, G. Jung,