| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9816879 | Ultramicroscopy | 2005 | 6 Pages |
Abstract
In this paper, a shear force scanning near-field fluorescence microscope combined with a confocal laser microspectrofluorometer is described. The shear force detection is realized based on a bimorph cantilever, which provides a very sensitive, reliable, and easy to use method to control the probe-sample distance during scanning. With the system, high-quality shear force imaging of various samples has been carried out. Furthermore, simultaneous shear force and near-field fluorescence imaging of biological cells has also been realized. As an example, we especially present the result on the distribution of P-glycoprotein in the plasma membrane of human small cell lung cancer cells, suggesting that the system would be a promising tool for biological applications.
Related Topics
Physical Sciences and Engineering
Materials Science
Nanotechnology
Authors
G.Y. Shang, W.H. Qiao, F.H. Lei, J.-F. Angiboust, M. Troyon, M. Manfait,