The recognition of biological cells utilizing quantitative phase microscopy system

Because computer recognition strongly depends on the quality of an image, the optical imaging system is of crucial importance. For this purpose one of the best solutions could be the Quantitative Phase microscopy (QPm) technique. QPm is the recent digital technique of phase contrast microscopy, providing quantitative phase and intensity data obtained from a series of defocused images. The following phase contrast modalities may be generated digitally from the computed QPm phase data with a greater degree of flexibility: Pure phase images (intensity-free), standard phase contrast (e.g. Zernike phase contrast), differential interference contrast (DIC), Hoffman modulation contrast, and simulated darkfield.