| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9879449 | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | 2005 | 8 Pages |
Abstract
The aim of this study was to compare rat tissues with respect to their reactive oxygen and nitrogen species (RONS) generating activities as a function of age. We quantified the RONS generation in vivo in young (6 months) and in old (30 months) male Sprague-Dawley rats using the recently developed spin trap 1-hydroxy-3-carboxy-pyrrolidine, applied intravenously. This spin trap reacts with superoxide radical and peroxynitrite yielding a stable spin adduct which is detectable by means of electron paramagnetic resonance (EPR) spectroscopy in frozen tissues. In old rats RONS generation was significantly increased compared to their young counterparts in the following order: blood
Keywords
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Biochemistry, Genetics and Molecular Biology
Ageing
Authors
Andrey V. Kozlov, Laszlo Szalay, Fraz Umar, Karl Kropik, Katrin Staniek, Hans Niedermüller, Soheyl Bahrami, Hans Nohl,