Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9881231 | Mechanisms of Ageing and Development | 2005 | 6 Pages |
Abstract
This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rbâ/â background were generated by crossings PhCMV-tTA/TRE-Rb transgenic mice with conventional Rb+/â mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rbâ/â embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.
Related Topics
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Biochemistry, Genetics and Molecular Biology
Ageing
Authors
Yoon Sik Choi, Jae Eun Lee, Cheolho Cheong, Young Hoon Sung, Eun Young Yang, Chan Bae Park, Jaewhan Song, Sang Chul Park, Han-Woong Lee,