Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9901460 | European Journal of Pharmaceutics and Biopharmaceutics | 2005 | 12 Pages |
Abstract
The commercially available reconstructed human epidermis models EpiSkin®, SkinEthic® and EpiDerm® demonstrate reasonable similarities to the native human tissue in terms of morphology, lipid composition and biochemical markers. These models have been identified as useful tools for the testing of phototoxicity, corrosivity and irritancy, and test protocols have been developed for such applications. For acceptance of these tests by the authorities, prevalidation or validation studies are currently in progress. Furthermore, first results also indicate their suitability for transport experiments of drugs and other xenobiotics across skin. Still, however, the barrier function of these reconstructed human epidermis models appears to be much less developed compared to native skin. Further adaptation of the models to the human epidermis, especially concerning the barrier function, therefore remains an important challenge in this area of research.
Keywords
SPRRHPTLCmRNASLSUVATLCSodium laurylsulfate3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromidestratum granulosumMTTO/Wmessenger RNAW/O/WReconstructed human epidermisinterleukinDrug deliveryLipid compositionlactate dehydrogenaseLDHStratum corneumBiochemical markersoil/waterSkinthin layer chromatographyhigh performance thin layer chromatography
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Frank Netzlaff, C.-M. Lehr, P.W. Wertz, U.F. Schaefer,