| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9902243 | Journal of Immunological Methods | 2005 | 5 Pages |
Abstract
IL-18 is an important cytokine in both innate and acquired immunity. IL-18 promoter polymorphisms have been investigated in several autoimmune and infectious diseases. Here we present two assays for a medium to a high throughput genotyping of C-607A and G-137C IL-18 SNPs. Using these protocols we have been able to rapidly genotype a cohort of 131 healthy Italian individuals. The assays, based on a multiplex allele-specific PCR and the melting temperature analysis of the PCR product, are rapid, easily automated, inexpensive and well suited to case-control association studies.
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Authors
Michele Boniotto, Ludovica Segat, Michele Milanese, Sergio Crovella,