| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9902248 | Journal of Immunological Methods | 2005 | 10 Pages |
Abstract
In conclusion, positive selection of CD56+ cells by Microbeads is better than Dynabeads, as determined from cell yield and procedure-associated cell activation and should be chosen for in vitro studies of NK/NKT cells. However, when pure NK cells and phenotypic subtypes are to be studied, negative sorting seems most appropriate.
Keywords
PBLsPBSFITCDynabeadsTCrICAM-1MicrobeadsPBMCsHBSSIFN-γinterferon-γELISAEnzyme-linked immunosorbent assaytumour necrosis factor-αNK cellNKT cellnatural killer T cellNatural killer cellperipheral blood mononuclear cellsTNF-αphycoerythrinfluorescein isothiocyanateperipheral blood leukocytesPhosphate-buffered salineHanks' balanced salt solutionintracellular adhesion molecule-1T cell receptor
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Authors
Anita Ãren, Christina Husebø, Ann-Charlotte Iversen, Rigmor Austgulen,